Abstract

Circadian clocks coordinate mammalian behavior and physiology enabling organisms to anticipate 24-hour cycles. Transcription-translation feedback loops are thought to drive these clocks in most of mammalian cells. However, red blood cells (RBCs), which do not contain a nucleus, and cannot perform transcription or translation, nonetheless exhibit circadian redox rhythms. Here we show human RBCs display circadian regulation of glucose metabolism, which is required to sustain daily redox oscillations. We found daily rhythms of metabolite levels and flux through glycolysis and the pentose phosphate pathway (PPP). We show that inhibition of critical enzymes in either pathway abolished 24-hour rhythms in metabolic flux and redox oscillations, and determined that metabolic oscillations are necessary for redox rhythmicity. Furthermore, metabolic flux rhythms also occur in nucleated cells, and persist when the core transcriptional circadian clockwork is absent in Bmal1 knockouts. Thus, we propose that rhythmic glucose metabolism is an integral process in circadian rhythms.

https://doi.org/10.1038/s41467-020-20479-4

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Abstract

Previous studies have shown the effect of extremely low-frequency (ELF) magnetic fields on the hematopoietic system. However, molecular modification and biological toxicity are not known yet. The aim of this study was to investigate the effect of occupational exposure to ELF magnetic field on the hemoglobin and DNA alteration using Fourier transform infrared (FTIR) spectroscopy. Twenty nine individuals were selected among those working as the controller in a powerhouse in order to be studied as the population exposed to ELF magnetic field. Control group comprised of 29 administrative employees voluntarily participated who were matched with the exposed subjects in terms of sex, age, work experiences, smoking habit, and socioeconomic status. DNA and hemoglobin were extracted from blood samples and then were studied by FTIR spectroscopy. The results showed the level of magnetic field exposure was between 0.38 to 50 μT in the exposed subjects while the level of magnetic field exposure was between 0.19 and 20 μT for the unexposed people. Hemoglobin level was equal to 15.67 ± 1.42 g/dL for exposed subjects which is significantly lower than that of the unexposed people (p = 0.0001). There was a significant alteration in CH content and COO structure of the hemoglobin structure. Moreover, DNA showed significant changes by functional group of organic base. This change in the structure of DNA and hemoglobin can lead to the creation of risks in human health. In conclusion, FTIR method could reveal the quality of DNA and hemoglobin structure in subjects after exposure to ELF magnetic field.

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