DNA Repair Archives - EMFSA https://www.emfsa.co.za/tag/dna-repair/ Electromagnetic fields South Africa Thu, 28 Oct 2021 18:53:45 +0000 en-US hourly 1 https://wordpress.org/?v=7.1.3 https://www.emfsa.co.za/wp-content/uploads/2021/02/cropped-EMFSA_logo-fv-32x32.png DNA Repair Archives - EMFSA https://www.emfsa.co.za/tag/dna-repair/ 32 32 Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and, apoptosis in pregnant mouse placenta https://www.emfsa.co.za/research-and-studies/wi-fi-2-4-ghz-affects-anti-oxidant-capacity-dna-repair-genes-expression-and-apoptosis-in-pregnant-mouse-placenta/ Thu, 30 Jul 2020 23:10:32 +0000 https://www.emfsa.co.za/?p=15402 Vafaei H, Kavari Gh, Izadi HR, Zare Dorahi Z, Dianatpour M, Daneshparvar A, Jamhiri I. Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and, apoptosis in pregnant mouse placenta. Iran J Basic Med Sci 2020; 23:833-840. doi: 10.22038/ijbms.2020.40184.9512 Abstract Objective(s): The placenta provides nutrients and oxygen to embryo and removes waste products from […]

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]]> Vafaei H, Kavari Gh, Izadi HR, Zare Dorahi Z, Dianatpour M, Daneshparvar A, Jamhiri I. Wi-Fi (2.4 GHz) affects anti-oxidant capacity, DNA repair genes expression and, apoptosis in pregnant mouse placenta. Iran J Basic Med Sci 2020; 23:833-840. doi: 10.22038/ijbms.2020.40184.9512

Abstract

Objective(s): The placenta provides nutrients and oxygen to embryo and removes waste products from embryo’s blood.

As far as we know, the effects of exposure to Wi-Fi (2.4 GHz) signals on placenta have not been evaluated. Hence, we examined the effect of prenatal exposure to Wi-Fi signals on anti-oxidant capacity, expressions of CDKNA1, and GADD45a as well as apoptosis in placenta and pregnancy outcome.

Materials and Methods: Pregnant mice were exposed to Wi-Fi signal (2.4 GHz) for 2 and 4 hr. Placenta tissues were examined to measure the MDA and SOD levels. To measure SOD, CDKNA1, GADD45a, Bax, and Bcl-2 expressions were compared by real-time PCR analysis. TUNEL assay was used to assess apoptosis in placenta tissues. The results were analyzed by one-way analysis of variance (ANOVA) using Prism version 6.0 software.

Results: MDA and SOD levels had significantly increased in exposed Wi-Fi signal groups (P-value< 0.05). Also, quantitative PCR experiment showed that SOD mRNA expression significantly increased in Wi-Fi signal groups. The data showed that CDKN1A and GADD45a genes were increased in Wi-Fi groups (P-value<0.05). The quantitative PCR and the TUNEL assay showed that apoptosis increased in Wi-Fi groups (P-value<0.05).

Conclusion: Our results provide evidence that Wi-Fi signals increase lipid peroxidation, SOD activity (oxidative stres), apoptosis and CDKN1A and GADD45a overexpression in mice placenta tissue.
However, further experimental studies are warranted to investigate other genes and aspects of pregnancy to determine the role of Wi-Fi radiation on fertility and pregnancy.

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]]> The role of radiation induced oxidative stress as a regulator of radio-adaptive responses https://www.emfsa.co.za/research-and-studies/the-role-of-radiation-induced-oxidative-stress-as-a-regulator-of-radio-adaptive-responses/ Sun, 26 Jan 2020 20:52:05 +0000 https://www.emfsa.co.za/?p=12003 Mohsen Sisakht, Maryam Darabian, Amir Mahmoodzadeh, Ali Bazi, Sayed Mohammad Shafiee, Pooneh Mokarram & Zahra Khoshdel (2020) The role of radiation induced oxidative stress as a regulator of radio-adaptive responses, International Journal of Radiation Biology, 96:5, 561-576, DOI: 10.1080/09553002.2020.1721597 Abstract Purpose Various sources of radiation including radiofrequency, electromagnetic radiation (EMR), low- dose X-radiation, low-level microwave radiation and ionizing radiation (IR) are indispensable […]

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  • Mohsen Sisakht, Maryam Darabian, Amir Mahmoodzadeh, Ali Bazi, Sayed Mohammad Shafiee, Pooneh Mokarram & Zahra Khoshdel (2020) The role of radiation induced oxidative stress as a regulator of radio-adaptive responses, International Journal of Radiation Biology, 96:5, 561-576, DOI: 10.1080/09553002.2020.1721597

    • Abstract

    Purpose

    Various sources of radiation including radiofrequency, electromagnetic radiation (EMR), low- dose X-radiation, low-level microwave radiation and ionizing radiation (IR) are indispensable parts of modern life. In the current review, we discussed the adaptive responses of biological systems to radiation with a focus on the impacts of radiation-induced oxidative stress (RIOS) and its molecular downstream signaling pathways.

    Materials and Methods

    A comprehensive search was conducted in Web of Sciences, PubMed, Scopus, Google Scholar, Embase, and Cochrane Library. Keywords included Mesh terms of “radiation”, “electromagnetic radiation”, “adaptive immunity”, “oxidative stress”, and “immune checkpoints”. Manuscript published up until December 2019 were included.

    Results

    RIOS induces various molecular adaptors connected with adaptive responses in radiation exposed cells. One of these adaptors includes p53 which promotes various cellular signaling pathways. RIOS also activates the intrinsic apoptotic pathway by depolarization of the mitochondrial membrane potential and activating the caspase apoptotic cascade. RIOS is also involved in radiation-induced proliferative responses through interaction with mitogen-activated protein kinases (MAPks) including p38 MAPK, ERK, and c-Jun N-terminal kinase (JNK). Protein kinase B (Akt)/phosphoinositide 3-kinase (PI3K) signaling pathway has also been reported to be involved in RIOS-induced proliferative responses. Furthermore, RIOS promotes genetic instability by introducing DNA structural and epigenetic alterations, as well as attenuating DNA repair mechanisms. Inflammatory transcription factors including macrophage migration inhibitory factor (MIF), nuclear factor κB (NF-κB), and signal transducer and activator of transcription-3 (STAT-3) paly major role in RIOS-induced inflammation.

    Results

    In conclusion, RIOS considerably contributes to radiation induced adaptive responses. Other possible molecular adaptors modulating RIOS-induced responses are yet to be divulged in future studies.

    https://www.tandfonline.com/doi/full/10.1080/09553002.2020.1721597

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    ]]>         Is 5G safe? https://www.emfsa.co.za/videos/is-5g-safe/ Sat, 23 Nov 2019 08:43:58 +0000 https://www.emfsa.co.za/?p=11275 Steve Hart interviews Prof Dariusz Leszczynski about  5G Watch the video at the link below: https://www.nzptv.org.nz/videos/is-5g-safe?fbclid=IwAR2eUNCH45NzJQh26kflcnGIXahUlzSRl_8G4DdNnBQxZAm1jXMSnhBkIak

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    ]]>     Steve Hart interviews Prof Dariusz Leszczynski about  5G

    Watch the video at the link below:

    https://www.nzptv.org.nz/videos/is-5g-safe?fbclid=IwAR2eUNCH45NzJQh26kflcnGIXahUlzSRl_8G4DdNnBQxZAm1jXMSnhBkIak

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