Abstract

Background:

The radiation emitted from electromagnetic fields (EMF) can cause biological effects on prokaryotic and eukaryotic cells, including non-thermal effects.

Objective:

The present study evaluated the non-thermal effects of wireless fidelity (Wi-Fi) operating at 2.4 GHz part of non-ionizing EMF on different pathogenic bacterial strains (Escherichia coli 0157H7, Staphylococcus aureus, and Staphylococcus epidermis). Antibiotic resistance, motility, metabolic activity and biofilm formation were examined.

Material and Methods:

In this case-control, a Wi-Fi router was used as a source of microwaves and also bacterial cells were exposed to Wi-Fi radiation continuously for 24 and 48 hours. The antibiotic susceptibility was carried out using a disc diffusion method on Müller Hinton agar plates. Motility of Escherichia coli 0157H7 was conducted on motility agar plates. Cell metabolic activity and biofilm formation were performed using 3-(4, 5-Dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and crystal violet quantification, respectively.

Results:

The exposure to Wi-Fi radiation altered motility and antibiotic susceptibility of Escherichia coli 0157H7. However, there was no effect Wi-Fi radiation on antibiotic susceptibility of Staphylococcus aureus and Staphylococcus epidermis. On the other hand, the exposed cells, as compared to the unexposed control, showed an increased metabolic activity and biofilm formation ability in Escherichia coli 0157H7, Staphylococcus aureus and Staphylococcus epidermis.

Conclusion:

These results proposed that Wi-Fi exposure acted on bacteria in stressful manner by increasing antibiotic resistance and motility of Escherichia coli 0157H7, as well as enhancing biofilm formation by Escherichia coli 0157H7, Staphylococcus aureus and Staphylococcus epidermis. The findings may have implications for the management of serious diseases caused by these infectious bacteria.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820025/?fbclid=IwAR2ZweuyjWhJV7dLq0KnrowPFKTt3imHqxc2dV0mgd_q_vP7hoGB6wfIM4M

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Abstract

A substantial increase in the number of mobile phone base stations (MPBS) has been demonstrated in the world. This development has raised concerns with biological systems due to electromagnetic field and radiations. Antibiotic resistance is referred as “the silent tsunami facing modern medicine”. The aim of this in vitro study was to demonstrate the impact of these radiations transmitted by Mobile tower stations on microbial diversity in soil and antibiotic resistance pattern. Soil samples were taken from near four different base stations located in Dausa city, while control samples were taken far from stations. Isolation and identification of microorganisms was done using biochemical reactions and antibiotic resistance was observed. Chi-square test with Yates correction was applied to compare the pattern of antibiotic resistance. Stenotrophomonas maltophilia, Chryseobacterium Gleum, Kocuria Rosea were isolated and identified in soil samples collected near radiation exposed zone. Greater antibiotic resistance was observed in microbes present in soil near base stations compared to control. A statistical significant difference in pattern of antibiotic resistance was found with Nalidixic acid, and cefixime when used as antimicrobial agents. (P-value less than 0.05). Our findings suggest that mobile tower radiations can significantly alter the vital systems in microbes and turn them multidrug resistant (MDR) which is most important current threat to public health.

DOI: 10.1109/PEEIC.2018.8665432

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Image credits: Dr  Erica Mallery-Blythe

Interview after the presentation by UK expert, Dr Erica Mallery-Blythe on health and environmental impacts associated with exposure to electromagnetic radiation, with an especial focus on 5G, to an invited audience including Bath MP Wera Hobhouse, some Bath City Councillors and members of the public. Guildhall Bath – 5th July 2019

The post Dr Erica Mallery-Blythe presentation – Impacts of exposure to electromagnetic radiation & 5G appeared first on EMFSA.

Abstract:

Background:

The radiation emitted from electromagnetic fields (EMF) can cause biological effects on prokaryotic and eukaryotic cells, including non-thermal effects.

Objective:

The present study evaluated the non-thermal effects of wireless fidelity (Wi-Fi) operating at 2.4 GHz part of non-ionizing EMF on different pathogenic bacterial strains (Escherichia coli O157H7, Staphylococcus aureus, and Staphylococcus epidermis). Antibiotic resistance, motility, metabolic activity and biofilm formation were examined.

Material and Methods:

A Wi-Fi router was used as a source of microwaves and also bacterial cells were exposed to Wi-Fi radiation continuously for 24 and 48 hours. The antibiotic susceptibility was carried out using a disc diffusion method on Müller Hinton agar plates. Motility of Escherichia coli O157H7 was conducted on
motility agar plates. Cell metabolic activity and biofilm formation were performed using 3-(4, 5-Dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and crystal violet quantification, respectively.

Results:

The exposure to Wi-Fi radiation altered motility and antibiotic susceptibility of Escherichia coli O157H7. However, there was no effect Wi-Fi radiation on antibiotic susceptibility of Staphylococcus aureus and Staphylococcus epidermis.
On the other hand, the exposed cells, as compared to the unexposed control, showed an increased metabolic activity and biofilm formation ability in Escherichia coli O157H7, Staphylococcus aureus and Staphylococcus epidermis.

Conclusion:

These results proposed that Wi-Fi exposure acted on bacteria in stressful manner by increasing antibiotic resistance and motility of Escherichia coli O157H7, as well as enhancing biofilm formation by Escherichia coli O157H7, Staphylococcus aureus and Staphylococcus epidermis. The findings may have implications for the management of serious diseases caused by these infectious bacteria.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820025/

The post Evaluation of Wi-Fi Radiation Effects on Antibiotic Susceptibility, Metabolic Activity and Biofilm Formation by Escherichia Coli O157H7, Staphylococcus Aureus and Staphylococcus Epidermis appeared first on EMFSA.